Technique for the simultaneous ultrastructural demonstration of anterogradely transported horseradish peroxidase and an immunocytochemically identified neuropeptide.

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Abstract
In order to extend the information obtainable from ultrastructural studies of synaptic connectivity using either horseradish peroxidase tracing or immunocytochemistry alone, we have developed a method of combining these two procedures. Thus it has been possible to examine the characteristics of axon terminals of known origin forming synaptic contacts with cells of identified neuropeptide content.

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