Isolation and characterization of human reg protein produced in Saccharomyces cerevisiae
Open Access
- 15 October 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 272 (1-2) , 85-88
- https://doi.org/10.1016/0014-5793(90)80454-q
Abstract
reg was originally identified a a gene expressed during the regeneration of insulin‐producing pancreatic β‐cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144‐amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino‐terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of α‐helix and β‐sheet contents.Keywords
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