ISOLATION OF NITROSOMONAS IN PURE CULTURE

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Abstract
The development of a nitrifying enrichment culture in solution media formulated to be relatively free of particulate matter was investigated. Rapid and dispersed growth of Nitrosomonas was obtained in a medium buffered by phosphate and containing (NH4)2SO4 as a source of energy. Under suitable conditions the ratio of the number of cells of Nitrosomonas to that of heterotrophic bacteria present in the enrichment culture exceeded 250, and isolation of Nitrosomonas in pure culture by dilution techniques was accomplished with comparative ease.
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