Comparative Studies of Leaf Tissue 3Antirrhinum majusL

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Abstract
The ion relations of isolated mesophyll cells from Antirrhinum leaf tissue were compared with the corresponding properties of the freshly excised leaf tissue. Both systems showed active influxes of K+ and Cl−, and the permeabilities of the cells to K+ and to Cl− were similar to those of the leaf tissue. However, the contents of K+ and Cl− in the isolated cells were much less than in the leaf tissue, and the permeability of the cells to Na+ was much higher. The results suggested that the isolation procedure had some reversible effects on the transport properties of the cells and also some irreversible effects. Comparison with other results suggested that the magnitudes of these effects depend on the species of plant being used. It was concluded that isolated mesophyll cells can be useful in the study of the ion transport processes which occur in leaves, provided that certain precautions are taken in the isolation of the cells and in the conditions of their use.

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