Effect of Trypsin on the Infectivity of Sendai Virus Grown in Several Host Cells

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Abstract
As described in a previous study, Sendai virus formed plaques in chick embryo (CE) cell monolayers with the aid of trypsin incorporated into the agar overlay medium. The virus also formed plaqůes in monkey kidney (MR) cell monolayers without the aid of trypsin. Sendai virus grown in CE cells, as well as that grown in either of other stable cell lines tested, showed much lower plaque‐forming unit (PFU) titers in MK monolayers than in CE monolayers. However, a mild trypsin treatment of the virus grown in these cells enhanced its PFU titers in MK monolayers to the level obtained in CE monolayers. In spite of this marked infectivity enhancement, the enzyme treatment little affected the hemagglutinin titers of the virus. On the other hand, Sendai virus grown in embryonated eggs or MK cells showed similar PFU titers in both CE and MK monolayers and also showed no evidence of infectivity enhancement due to the enzyme treatment.