Transformation of Cultivated Alfalfa Using Disarmed Agrobacterium tumefaciens1
- 1 November 1986
- journal article
- research article
- Published by Wiley in Crop Science
- Vol. 26 (6) , 1235-1239
- https://doi.org/10.2135/cropsci1986.0011183x002600060033x
Abstract
This study was conducted to establish a transformation system for cultivated alfalfa (Medicago sativa L.), a highly valued forage legume. Genetic transformation of alfalfa ‘CUF101’ was carried out using Agrobacterium tumefaciens. A dominant selectable marker (Nos/ Npt) for kanamyycin resistance was introduced into alfalfa tissues via a disarmed vector (pARC8) residing in the bacterium. The Nos/ Npt gene was stably integrated into the plant genome and expressed in selected, kanamycin‐resistant calli and in regenerated plants. Transgenic alfalfa plants were also obtained using two different vectors derived from pARC8.This publication has 14 references indexed in Scilit:
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