Presence of 17-Ketosteroids in Adrenal Perfusates.

Abstract
Blood containing added ACTH and perfused through bovine adrenal glands, was purified and studied for its C19 steroid content. On paper chromatograms of the ethyl acetate extract of the blood perfusate 5 17-ketosteroids were detected. Further study of the behavior of the free 17-ketosteroids and their derivatives by paper chromatographic techniques involving several solvent systems and color tests allowed the identification of 3 compounds as 4-androstene-3, 17-dione, 4-androstene-3, 11, 17-trione and44-androstene-11B-ol-3, 17-dione.