Recombinational repair of alkylation lesions in phage T4
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 167 (2) , 185-195
- https://doi.org/10.1007/bf00266912
Abstract
Treatment of phage T4-host adsorption complexes by MNNG1 increased recombination between two rII markers by about three-fold. Temperature sensitive mutants defective in genes 32, 46 and 47, which cause reductions in recombination at semirestrictive temperatures, proved to be substantially more sensitive to MNNG at such temperatures than wild-type phage. In addition, the recombination defective mutants x m(uvsX) and y 10(y) were more sensitive to MNNG than wild-type, whereas mutants defective in genes 45 and denV, which are apparently not involved in recombination, were not MNNG sensitive. These findings suggest that a recombination pathway involving the products of genes 32, 46, 47, uvsX and y is employed in repairing MNNG-induced lethal lesions. This mechanism is effective in cells infected by single phage, implying post-replication recombinational repair between daughter chromosomes. MNNG-induced lesions are subject to multiplicity reactivation, but mutants defective in genes 46 or 47 showed the same degree of multiplicity reactivation as wild-type phage. The gene 32 and gene 47 recombination defective mutants were tested for their effects on MNNG-induced reversion of an rII marker. No reduction in induced reversion was found. Thus, it appears that the postulated recombinational repair pathway employing the products of genes 32 and 47 does not contribute substantially to induced mutagenesis.This publication has 82 references indexed in Scilit:
- Mechanism of potent mutagenic action of N-Methyl-N′-nitro-N-nitrosoguanidine on intracellular phage LambdaJournal of Molecular Biology, 1978
- Germ line recombination may be primarily a manifestation of DNA repair processesJournal of Theoretical Biology, 1977
- UV-induced mitotic recombination in somatic cells of Drosophila melanogasterMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1976
- Induction of mitotic recombination with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) in Saccharomyces cerevisiae. A comparison between treatment in vitro and in the host-mediated assayMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1976
- UV irradiation of polar cells of drosophila melanogaster embryos: V. A study of the meiotic recombination in females with chromosomes of different structureMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- Stimulation of mutation in phage T4 by lesions in gene 32 and by thymidine imbalanceMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- Role of genes 46 and 47 in bacteriophage T4 reproduction: II. Formation of gaps on parental DNA of polynucleotide ligase defective mutantsJournal of Molecular Biology, 1972
- Studies on the substrate specificity of the T4 excision repair endonucleaseMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- Specific excision of methylation products from DNA of Escherichia coli treated with N-methyl-N′-nitro-N-nitrosoguanidineChemico-Biological Interactions, 1970
- Endonucleolytic cleavage of UV-irradiated DNA controlled by the V+ gene in phage T4Biochemical and Biophysical Research Communications, 1969