A Method to Measure Pyruvate in Milk
- 1 November 1976
- journal article
- research article
- Published by International Association for Food Protection in Journal of Milk and Food Technology
- Vol. 39 (11) , 776-777
- https://doi.org/10.4315/0022-2747-39.11.776
Abstract
A simple procedure to measure pyruvate in fluid milk products was developed. A supernatant fluid from test milk was prepared by clotting with rennet followed by centrifugation. The supernatant fluid was then boiled centrifuged again, and finally membrane-filtered. This filtrate was then used in the spectrophotometric assay of pyruvate via the NADH NAD+ mediated conversion of pyruvate to lactate by lactate dehydrogenase (LDH). This procedure recovered nearly 100% of pyruvate added to various milks, and detected a difference of 1 ppm of pyruvate (p < 0.01).This publication has 0 references indexed in Scilit: