Coherence Selection by Gradients without Signal Attenuation: Application to the Three‐Dimensional HNCO Experiment
- 1 October 1993
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition in English
- Vol. 32 (10) , 1489-1491
- https://doi.org/10.1002/anie.199314891
Abstract
Maximum sensitivity and excellent suppression of the water signal is achieved in 3D triple‐resonance NMR experiments by the application of a heteronuclear gradient echo in phase‐modulating pulse sequences. This principle is demonstrated for an HNCO experiment applied to a complex of a uniformly 13C/15Nlabeled protein (calmodulin) and a peptide (C2OW).Keywords
This publication has 26 references indexed in Scilit:
- Gradient-enhanced heteronuclear correlation spectroscopy. Theory and experimental aspectsJournal of Magnetic Resonance (1969), 1992
- The generation of phase-sensitive 2D 15N1H spectra using gradient pulses for coherence-transfer-pathway selectionJournal of Magnetic Resonance (1969), 1992
- Pure-phase heteronuclear multiple-quantum spectroscopy using field gradient selectionJournal of Magnetic Resonance (1969), 1992
- Experiments for recording pure-absorption heteronuclear correlation spectra using pulsed field gradientsJournal of Magnetic Resonance (1969), 1992
- Efficient detection of CαH-HN correlations in proteins using gradient-enhanced 15N HMQC-TOCSYJournal of Magnetic Resonance (1969), 1991
- Absorption-mode two-dimensional NMR spectra recorded using pulsed field gradientsJournal of Magnetic Resonance (1969), 1991
- Gradient-enhanced spectroscopyJournal of Magnetic Resonance (1969), 1990
- Pulsed field gradients in NMR. An alternative to phase cyclingJournal of Magnetic Resonance (1969), 1985
- Selection of coherence-transfer pathways in NMR pulse experimentsJournal of Magnetic Resonance (1969), 1984
- A two-dimensional nuclear overhauser experiment with pure absorption phase in four quadrantsJournal of Magnetic Resonance (1969), 1982