STABLE ISOLATED SYMMETRICAL ANHYDRIDES OF N-ALPHA-9-FLUORENYLMETHYLOXYCARBONYLAMINO ACIDS IN SOLID-PHASE PEPTIDE-SYNTHESIS - METHIONINE-ENKEPHALIN SYNTHESIS AS AN EXAMPLE
- 1 January 1981
- journal article
- research article
- Vol. 18 (3) , 237-241
Abstract
The synthesis and isolation of symmetrical anhydrides of N.alpha.-9-fluorenylmethyloxycarbonyl (Fmoc) amino acids using water soluble carbodiimide is described. These compounds were used in a solid phase peptide synthesis of methionine-enkephalin on a p-benzyloxybenzyl ester polystyrene 1% divinylbenzene resin support. Homogeneous free pentapeptide was obtained in 42% overall yield. The Fmoc amino acid symmetrical anhydrides were stable during prolonged storage (2 yr at 0.degree.) and offer advantages over present Fmoc solid phase methods which use anhydrides formed in situ.This publication has 9 references indexed in Scilit:
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