In situ DNA sequence mapping with surface-spread mouse pachytene chromosomes
- 1 January 1990
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 53 (4) , 219-220
- https://doi.org/10.1159/000132935
Abstract
Surface-spread pachytene chromosomes are several times the length of metaphase chromosomes and the decondensed chromatin loops are attached to a well-defined axis (Weith and Traut, 1980). This arrangement permits detailed DNA sequence localization by in situ hybridization. We show that two probes to low-frequency repeated sequences (20 to 50 copies) which locate the centromere proximal in the mouse X metaphase chromosome between bands Al and A3 (Disteche et al., 1985) and which map 5.5 cM apart (Disteche et al., 1989), hybridize to two distinct chromatin regions 3 to 5 µm apart on a 25 µm long pachytene X chromosome core.Keywords
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