Über die Komponenten der Bernsteinsäureoxydase

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Abstract
Cytochrome c was prepared from horse heart muscle by the method of Keilin and Hartree (Proc. Roy Soc. B, 122: 298, 1937). The cholate prepn. was obtained by extracting pig heart muscle with M/10 phosphate buffer, pH 7.4, and treating the soln. with 1/4 its vol. of 10% Na cholate soln. The cholate prepn. was precipitated from the soln. by (NH4)2SO4. The cholate preparation contained cytochrome oxidase, cytochrome a, and succinodehydrogenase and small amts. of cytochromes c and b, and malic acid dehy-drogenase. The SC factor (succinodehydrogenase-cyto-chrome c combining factor) was prepared by extracting pig heart muscle with the phosphate buffer, adjusting the pH to 9 with NaOH, and heating the soln. to 55[degree] for 15 min. The soln. was cooled and neutralized. Cytochrome c, the cholate prepn. and the SC factor were required to oxidize succinic acid in the presence of oxygen. The SC factor catalyzed the reaction between the dehydrogenase and cytochrome c, but was inactive between diaphorase and cytochrome c. The SC factor was also required for the reduction of cytochrome a. The suggested mechanism for the reduction was: Succinodehydrogenase [forward arrow] SC factor [forward arrow] cytochrome c [forward arrow] cytochrome a [forward arrow] cytochrome oxidase.

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