Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.
- 15 March 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (6) , 2456-2460
- https://doi.org/10.1073/pnas.89.6.2456
Abstract
A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.Keywords
This publication has 25 references indexed in Scilit:
- Isolation of Single-Copy Human Genes from a Library of Yeast Artificial Chromosome ClonesScience, 1989
- Genetic analysis of the mouse using interspecific crossesTrends in Genetics, 1988
- Transformation of yeast spheroplasts without cell fusionAnalytical Biochemistry, 1987
- Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome VectorsScience, 1987
- Separation of Large DNA Molecules by Contour-Clamped Homogeneous Electric FieldsScience, 1986
- GERM-LINE TRANSFORMATION OF MICEAnnual Review of Genetics, 1986
- Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate geneNature, 1986
- Knobbly, a new dominant mutation in the mouse that affects embryonic ectoderm organizationGenetics Research, 1984
- Dispersion of α-like globin genes of the mouse to three different chromosomesNature, 1981
- A herpes simplex virus 1 integration site in the mouse genome defined by somatic cell genetic analysisCell, 1978