Incubation of the Completed Kahn Test

Abstract
When the completed Kahn test is incubated overnight at room, icebox, or incubator temp., the character of the reactions varies materially from those obtained in reading the test in the standard method. Incubation of Kahn tests overnight at icebox temp. tends to give false positive reactions. Overnight incubation at 37.5[degree] C. tends to dissolve formed precipitates and to give negative reactions. Incubation at room temp. has comparatively little effect on Kahn reactions. It appears that most reliable results are obtained when the Kahn test is read directly after the 3-min. shaking period.

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