A simple analysis of 5 thinner components in human body fluids by headspace solid-phase microextraction (SPME)

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Abstract
A simple method for the extraction of 5 thinner components from human whole blood and urine, using the headspace solid-phase microextraction (SPME) method is presented. After heating a vial containing the samples with 5 compounds (toluene, benzene,n-butyl acetate,n-butanol andn-isoamyl acetate) at 80°C, a polydimethylsiloxane-coated SPME fiber was exposed to the headspace of the vial to allow adsorption of the compounds. The fiber needle was then injected into a capillary gas chromatography (GC) port. The headspace SPME-GC gave intense peaks for each compound and a low level of background noise was seen only for whole blood. Recovery rates o the 5 compounds by use of the headspace SPME-GC were 50–70%. Reproducibility for headspace SPME-GC data were excellent for both body fluids. The calibration curves showed linearity in the range 2–100 ng/0.5 ml whole blood or urine. The detection limits of each compound were 1.1–2.4 ng/0.5 ml sample. The present results on the analysis of 5 thinner components by headspace SPME-GC suggest its applicability to a number of other volatile compounds in forensic toxicology.