Susceptibility ofBacillus Larvaeto Ethylene Oxide and Tetracycline Hcl

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Abstract
Isolates of Bacillus larvae spores obtained from various geographical areas in the USA were surveyed as to susceptibility to tetracyline and to ethylene oxide (ETO). No evidence of resistance to tetracycline HCI was found, nor was there any difference in the resistance of isolates collected before and after tetracyclines were used to control bee diseases. When B. larvae spores from 28 of these sources were treated with ETO (450 mg. litre at 38°C and 50% RH), more than 99% of the spores were killed by a 60-min exposure, and only chance growth occurred after a 90-min exposure. There was no evidence of significant variations in the suceptibility of the various isolates of B. larvae to ETO.