Resistance of Human Hepatitis Delta Virus RNAs to Dicer Activity
- 15 November 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (22) , 11910-11917
- https://doi.org/10.1128/jvi.77.22.11910-11917.2003
Abstract
The endonuclease dicer cleaves RNAs that are 100% double stranded and certain RNAs with extensive but <100% pairing to release ∼21-nucleotide (nt) fragments. Circular 1,679-nt genomic and antigenomic RNAs of human hepatitis delta virus (HDV) can fold into a rod-like structure with 74% pairing. However, during HDV replication in hepatocytes of human, woodchuck, and mouse origin, no ∼21-nt RNAs were detected. Likewise, in vitro, purified recombinant dicer gave 80% cleaved in vitro. Other 66-nt hairpins derived from one end of genomic HDV, PSTVd, or ASBVd RNAs were also cleaved. Apparently, for unit-length RNAs of HDV, PSTVd, and ASBVd, it is the extended structure with <100% base pairing that confers significant resistance to dicer action.Keywords
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