Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid
- 19 August 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 188 (1) , 33-36
- https://doi.org/10.1016/0014-5793(85)80869-3
Abstract
A cDNA library from rat brain was constructed in pBR322 and screened with a 14‐mer mixed oligonucleotide probe based on residues 231–235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334‐base‐pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.Keywords
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