Rapid visual detection of microorganisms in blood culture

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Abstract
A method and apparatus for rapid visual detection of microorganisms in blood cultures are described. In the 30 min procedure, a lysing reagent for the preferential digestion of blood cells and a concentration device which causes 1 ml of lysed specimen to pass through a portion (3 mm2) of a membrane filter (pore size, 0.6 .mu.m) were used. After the material remaining on the filter was Gram stained, the filter was mounted and examined microscopically. The ability to detect microorganisms in blood cultures was determined by spiking 7 common blood pathogens into blood cultures prepared from the blood of health volunteers. Microorganism concentration in the cultures ranged from 1-1000/ml. All of 34 cultures with at lest 100 CFU[colony-forming units]/ml were detected, 34 of 64 cultures with < 100 CFU/ml were detected and 41 of 42 negative controls were correctly reported as negative.