CONVERSION OF α-KETO-ISOCAPROIC ACID TO ISO-AMYL ALCOHOL BY YEAST PYRUVATE DECARBOXYLASE AND ALCOHOL DEHYDROGENASE
Open Access
- 8 July 1969
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 75 (4) , 359-363
- https://doi.org/10.1002/j.2050-0416.1969.tb03226.x
Abstract
Brewer's yeast growing at a fixed rate in a chemostat in nitrogen-limiting medium with leucine as nitrogen source produces about 100 times as much isoamyl alcohol as a culture with (NH4)2SO4 as nitrogen source. The maximum rate of decarboxylase activity towards α keto-isocaproate is, however, the same for the two cultures, for a given maximum rate towards pyruvate. The control of isovaleraldehyde production is solely through the supply of α keto-isocaproate. While alcohol dehydrogenase and nucleotide levels are the same for the two cell-free extracts, those of the leucine-grown cells show 2–3 times as great alcohol dehydrogenase activity towards isovaleraldehyde as those from cells grown on (NH4)2SO4.This publication has 6 references indexed in Scilit:
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