Decline in Energy Reserves of Chlorella sorokiniana upon Exposure to Ozone

Publisher Website
Google Scholar
Abstract
Ozone exposure increases the loss of K from Chlorella cells due to an increase in passive permeability and a depolarization of membrane potential. One factor which likely influences or can be influenced by these changes is the energy state of the cell. Various indicators of cell energy status were examined in the presence and absence of O3. The active uptake of chloride and deoxyglucose is nearly completely inhibited by O3 at a dose at which cellular death, measured by plating efficiency, is minimal. Glucose-stimulated respiration, dependent upon ATP/ADP balance, is depressed to a greater degree than endogenous respiration in ozonated cells. Total ATP and G-6-P levels also decrease but not as rapidly, and labeled intermediates of glucose metabolism are lost. Thus, exposure to O3 results in a depletion of the cell''s energy reserves as substantiated by changes observed in processes which both utilize and generate ATP. This loss in energy reserves occurs at the same exposure level of O3 as do the changes in passive transport properties. The processes seem related to O3 injury.