2D crystal forms of annexin IV on lipid monolayers

Abstract
Two-dimensional crystalline arrays of annexin IV were generated by interaction of the purified protein with a phospholipid monolayer. Image analysis of electron micrographs of the protein crystals, which diffracted to 3.5 nm respectively, revealed p6 and p3 symmetry. Annexin IV gave two crystal forms with unit cells of 18 × 18 nm and 28 × 28 nm. The former unit cells wax similar to a previously described form of annexin VI. The implications of those observations are discussed.