CHARACTERIZATION AND LOCALIZATION OF CAMP-DEPENDENT PROTEIN-KINASES IN RAT CAUDAL EPIDIDYMAL SPERM

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 259  (2) , 832-838
Abstract
The subcellular localization of type I and type II cAMP-dependent protein kinases in rat caudal epididymal sperm was studied. A large fraction of the cAMP-binding activity (.apprx. 60%), as well as a significant portion of the catalytic activity (.apprx. 40%), remained associated with insoluble material after the plasma membrane was extracted with 1% Triton X-100. Only 15% of the cAMP-binding activity in sperm was membrane bound. To explore the possibility of a surface location for the membrane-associated cAMP-binding proteins, whole sperm were subjected to proteolysis. Sperm treated with .alpha.-chymotrypsin lost cAMP-binding activity in cytoplasmic, detergent-soluble, and detergent-resistant fractions. .alpha.-Chymotrypsin evidently had access to intracellular locations and to all of the cAMP-binding proteins in sperm. Photoaffinity labeling studies using 8-azido-[32P]cAMP suggest that the regulatory subunit (R) present in the membrane was predominantly RI, while the major species present in the cytoplasm and on detergent-resistant structures was RII. Detergent-resistant sperm structures could be depleted of endogenous catalytic activity by the addition of cAMP, and cAMP-dependent kinase activity could be subsequently regained by the addition of bovine heart catalytic subunits. Separation of head and tailpieces showed that the detergent-resistant regulatory subunits were almost exclusively located on the tailpiece. The involvement of cAMP-dependent protein kinases in sperm motility was considered.

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