Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs.
- 1 April 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (8) , 2571-2575
- https://doi.org/10.1073/pnas.83.8.2571
Abstract
The leaf disc tranformation system provides a simple means to score expression of various T-DNA markers within days of infection by A. tumefaciens as well as long-term selection for growth of transformed callus and shoots. In this report, we describe the application of this system to evaluation of marker transfer and integration in a comprehensive set of defined avirulent mutants of A. tumefaciens. We conclude that virC is not essential when the T-DNA is present on a binary vector. The most likely explanation for this is that the virC gene product is involved in generation of a T-DNA intermediate.This publication has 19 references indexed in Scilit:
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