Accumulation of Phenolic Compounds and Phytoalexins in Sliced and Elicitor-Treated Cotyledons of Cicer arietinum L.

Abstract
Upon slicing cotyledons of chickpea, Cicer arietinum L.. accumulate the pterocarpan phyto­alexins medicarpin and maackiain. Treatment of this tissue with an elicitor from the phytopathogcnic deuteromyccte Ascochyta rabiei (Pass.) Lab. greatly enhances accumulation of the pterocarpans and of other isoflavones and flavonoids. Isolation, chromatographic purification and structural elucidation by spectroscopic techniques of 16 phenolic compounds is described. Cotyledons induced for phytoalexin biosynthesis readily accumulate the isoflavones daidzein, formononetin, calycosin and pseudobaptigenin which are thought to be intermediates in pterocarpan formation.

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