Preliminary assessment of a multiwire camera for quantitative autoradiography of tritium-labelled substances
- 1 May 1986
- journal article
- research article
- Published by IOP Publishing in Physics in Medicine & Biology
- Vol. 31 (5) , 535-542
- https://doi.org/10.1088/0031-9155/31/5/006
Abstract
A multiwire camera is assessed for the high speed, quantitative autoradiography of tritium-labelled substances in two-dimensional systems. Exposure times for 3H were typically 1000 times less than film-based methods, with a detection efficiency of 19+or-3% for those beta particles predicted to escape into the sensitive region from uniformly 3H-labelled plastic segments of 30 mu m thickness. The spatial resolving power was calculated to be 0.4 mm full width half maximum (FWHM), the background count rate was 1 CPM cm-2 and the lowest activity monitored for a biochemical study was 0.15 Bq cm-2. The camera is expected to have wide applications for detecting and quantifying biological substances in a wide range of separation media.This publication has 16 references indexed in Scilit:
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