Growth retardation and increased apoptosis in mice with homozygous disruption of the akt1 gene
Top Cited Papers
Open Access
- 1 September 2001
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 15 (17) , 2203-2208
- https://doi.org/10.1101/gad.913901
Abstract
The serine/threonine kinase Akt has been implicated in the control of cell survival and metabolism. Here we report the disruption of the most ubiquitously expressed member of theakt family of genes, akt1, in the mouse. Akt1−/− mice are viable but smaller when compared to wild-type littermates. In addition, the life span of Akt1−/− mice, upon exposure to genotoxic stress, is shorter. However, Akt1−/− mice do not display a diabetic phenotype. Increased spontaneous apoptosis in testes, and attenuation of spermatogenesis is observed in Akt1−/− male mice. Increased spontaneous apoptosis is also observed in the thymi of Akt1−/− mice, and Akt1−/− thymocytes are more sensitive to apoptosis induced by γ-irradiation and dexamethasone. Finally, Akt1−/− mouse embryo fibroblasts (MEFs) are more susceptible to apoptosis induced by TNF, anti-Fas, UV irradiation, and serum withdrawal.Keywords
This publication has 41 references indexed in Scilit:
- The conserved PI3′K/PTEN/Akt signaling pathway regulates both cell size and survival in DrosophilaOncogene, 2000
- Protein Kinase B Regulates T Lymphocyte Survival, Nuclear Factor κb Activation, and Bcl-XL Levels in VivoThe Journal of Experimental Medicine, 2000
- Drosophila PTEN Regulates Cell Growth and Proliferation through PI3K-Dependent and -Independent PathwaysDevelopmental Biology, 2000
- Kit/stem cell factor receptor-induced activation of phosphatidylinositol 3′-kinase is essential for male fertilityNature Genetics, 2000
- The Regulation and Activities of the Multifunctional Serine/Threonine Kinase Akt/PKBExperimental Cell Research, 1999
- Heregulin Regulation of Akt/Protein Kinase B in Breast Cancer CellsBiochemical and Biophysical Research Communications, 1999
- Negative Regulation of PKB/Akt-Dependent Cell Survival by the Tumor Suppressor PTENCell, 1998
- Potential Role of Protein Kinase B in Glucose Transporter 4 Translocation in AdipocytesEndocrinology, 1997
- p53 is required for radiation-induced apoptosis in mouse thymocytesNature, 1993
- Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogeneCell, 1991