Studies on the Low Temperature Fermentation

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Abstract
With the Brevibacterium sp. P145 isolated from soil, the effect of incubation temperature on the extracellular amino acid accumulation has been examined from cultural and enzymological points of view. The strain was found to accumulate L-glutamic acid up to 5.88 mg/ml and L-alanine 0.38 mg/ml at 5°C, whereas it formed 0.21 mg/ml of L-glutamic acid and 2.54 mg/ml of L-alanine at 28°C. The accumulation of L-alanine in the medium at 28°C seemed to be related to the thiamine requirement of the strain. In the case of thiamine deficiency, L-alanine was the main product in the culture at 28°C. When the incubation temperature was abruptly shifted from 28°C to 5°C or from 5°Cto 28°C, the amino acid accumulation was also changed to that of the final temperature. L-Alanine dehydrogenase existed even in the cells grown at 5°C but was not active at this low temperature. These results were in accord with the informations obtained from cultural experiments.

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