The GC‐selective ligand mithramycin alters the structure of (AT)n sequences flanking its binding sites
- 7 May 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 264 (1) , 100-104
- https://doi.org/10.1016/0014-5793(90)80775-e
Abstract
DNA fragments containing (AT)n inserts cloned adjacent to putative mithramycin binding sites have been examined by footprinting experiments using a variety of nucleases in the presence of the drug. The results demonstrate that mithramycin induces a DNA structural change which renders adjacent (AT)n sequences sensitive to attack by DNase II. Significant changes are also revealed with DNase I and micrococcal nuclease. The results are consistent with a model in which mithramycin opens the DNA minor groove changing it to a structure which is locally more like A‐DNA.Keywords
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