Magnitude of a finite equilibration effect in analytical gel chromatography
- 1 October 1972
- journal article
- research article
- Published by Wiley in Biopolymers
- Vol. 11 (10) , 2125-2130
- https://doi.org/10.1002/bip.1972.360111012
Abstract
Previous analyses of solute behavior in analytical gel chromatography1,2 have allowed for a finite equilibration time between stationary and mobile phases in estimating the magnitude of axial dispersion coefficients, but have ignored these effects in formulating the continuity equations for solute transport. We develop a more accurate theory including the equilibration effect, and show experimentally that it can be disregarded after approximately two minutes from the start of a small‐zone experiment.Keywords
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