THE ISOLATION OF OLIGOSACCHARIDES FROM THE CELL-WALL POLYSACCHARIDE OF LACTOBACILLUS CASEI, SEROLOGICAL GROUP C

Publisher Website
Google Scholar
Abstract
A number of disaccharides and oligosaccharides have been isolated from the products of mild acid hydrolysis of the specific substance from L. casei, serological group C. The major disaccharide is O-[beta]-D-glucopyranosyl-(l[forward arrow]3)-N-acetyl-D-galactosamine (B4) and evidence is presented for the structure of a tetrasaccharide composed of O-[beta]-D-glucopyranosyl-(l 6)-D-galactose (Bl) joined through its reducing end group to B4. Disaccharide Bl is also a component of a trisaccharide 0-[beta]-D-glucopyranosyl-(l[forward arrow]6)-O-[beta]-D-galactopyranosyl-(1[forward arrow]6)-N-acetyl-D-glucosamine (A7). A number of other oligosaccharides have been shown to be related structurally. The ability of certain of the oligosaccharides to inhibit the precipitin reaction has been studied. The disaccharide Bl is more effective as an inhibitor than gentiobiose and the trisaccharide A7 is considerably more effective than Bl. These results have been compared with those obtained previously for the composition of the cell wall.