Detection of Thiols and Disulfides in Liver Samples using Liquid Chromatography/Electrochemistry
- 1 March 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 8 (4) , 691-706
- https://doi.org/10.1080/01483918508067113
Abstract
The determination of thiols and disulfides independent of one another is accomplished through the use of dual electrode liquid chromatography/electrochemistry and N-ethylmaleimide. The method can be used to assess peak purity as well as to determine the oxidation state of an unknown thiol. By the addition of NEM, trace disulfides can be determined without interferences from glutathione or other thiols present in much higher concentrations in in the liver. This method is used for the detection of mixed disulfides in liver samples.This publication has 18 references indexed in Scilit:
- Analysis of hepatic reduced glutathine, cysteine and homocysteine by cation-exchange high-performance liquid chromatography with electrochemical detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1984
- Rapid automated determination of d-penicillamine in plasma and urine by ion-exchange high-performance liquid chromatography with electrochemical detection using a gold electrodeJournal of Chromatography A, 1981
- Estimation of glutathione in rat liver by reversed-phase high-performance liquid chromatography: separation from cysteine and γ-glutamylcysteineJournal of Chromatography A, 1980
- Determination of reduced glutathione in guinea pig and rat tissue by HPLC with electrochemical detectionLife Sciences, 1978
- Mercury-based electrochemical detector for liquid chromatography for the detection of glutathione and other sulfur-containing compoundsAnalytical Chemistry, 1977
- High Pressure Liquid Chromatography of Selected Sulfur CompoundsAnalytical Letters, 1977
- A fluorometric method for determination of oxidized and reduced glutathione in tissuesAnalytical Biochemistry, 1976
- Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissuesAnalytical Biochemistry, 1969
- Tissue sulfhydryl groupsArchives of Biochemistry and Biophysics, 1959
- THE DETERMINATION OF BLOOD GLUTATHIONEPublished by Elsevier ,1932