Effect of Detergent on “Promiscuous” Inhibitors
- 1 July 2003
- journal article
- letter
- Published by American Chemical Society (ACS) in Journal of Medicinal Chemistry
- Vol. 46 (16) , 3448-3451
- https://doi.org/10.1021/jm0340896
Abstract
The term “promiscuous” inhibitors has been coined for compounds whose inhibition mechanism involves the interaction of aggregates of many compound molecules with the target protein, rather than the binding of individual molecules. This paper demonstrates that promiscuous inhibitors can be differentiated from classical 1:1 inhibitors by the judicious use of detergents, making it possible to configure assays that significantly reduce this undesirable mechanism of inhibition without compromising assay performance.Keywords
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