Regulation of Homocytotropic Antibody Formation in the Rat

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Abstract
Homocytotropic antibody (HTA) formation, as measured by the passive cutaneous anaphylaxis (PCA) test, was enhanced, although after a delay, and prolonged by 400 R whole body x-irradiation given shortly before through 8 days after immunization of rats with dinitrophenylated Ascaris extracts (DNP-As) plus killed Bordetella pertussis. Delayed enhancement and prolonged production were most striking when x-irradiation was given 1 day before to 2 days after initial immunization. An adjuvant effect of 400 R was produced in rats immunized with DNP-As alone omitting B. pertussis. As evidenced by passive hemagglutinin titers after 2-ME treatment, the production of γG antibody was greatly suppressed by 400 R x-irradiation. Two possible pathways to account for the irradiation effects on HTA formation were postulated, i.e., the enhancement of γM antibody and the suppression of 7S γG antibody resulted in a defective humoral feed-back regulation, and the depletion of certain populations of lymphoid cells, probably of thymic origin, caused cellular regulatory disturbances.

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