Bordetella pertussis Culture Having Only Species Factor 1

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Abstract
Preston''s description of Bordetella pertusis 353-Z as a culture possessing only factor 1 was confirmed by agglutination and agglutinin-adsorption tests. The LD50 values of this culture for mice inoculated by the intracerebral route were approximately the same as that of B. pertussis 18-323, the challenge culture in mouse potency tests of pertussis vaccine. In mouse protection tests vaccine prepared with the factor 1 culture protected as well against challenge with B. pertussis 18-323. which has a broad antigenic pattern, as did vaccines made with cultures possessing factors 2, 3, 4, and 5 in addition to factor 1. When 2 groups of similarly immunized mice were challenged with 353-Z and 18-323, respectively, much lower ED50 values were obtained with the animals challenged with 353-Z. This was true whether a factor 1 vaccine or a vaccine with factors 1, 2, 3, 4, and 5 was tested.