Cloning and Amplified Expression of the Tyrosyl-tRNA Synthetase Genes of Bacillus stearothermophilus and Escherichia coli

Open Access
Publisher Website
Google Scholar
Abstract
Two fragments of DNA which carry the genes coding for the tyrosyl‐tRNA synthetases of Escherichia coli and Bacillus stearothermophilus have been cloned into the plasmid pBR322 and were selected by complementation of an E. coli temperature‐sensitive mutant. Transformation of this strain with either of the recombinant plasmids results in a 100‐fold increase in tyrosyl‐tRNA synthetase activity measured in vitro and the protein products co‐migrate with the corresponding purified enzymes on polyacrylamide gels.