Biochemical characteristics of Chuzan virus, a new serotype of Palyam serogroup.

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Abstract
Biochemical properties of a Chuzan virus, strain K-47, being suspected as an etiologic agent for congenital malformation in cattle, were studied. The K-47 strain was shown to contain a double-stranded ribonucleic acid (RNA) genome consisting of ten segments and molecular weights ranging from 2.35 .times. 106 to 0.32 .times. 106 daltons by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Assuming only one RNA molecule per gel band, the total genome size was estimated to be 11.75 .times. 106 daltons. The structural polypeptides of viral particles were analyzed by radioimmunoprecipitation. Of the ten polypeptides ranging from 23K to 130K resolved by SDS-PAGE, three bands (95K, 86K and 23K) seem to be major structural polypeptides. The Mg2+-dependent RNA polymerase activity with the K-47 strain was demonstrated in an in vitro system, and was not affected by actinomycin D.