EFFECT OF TERBUTALINE ON MOVEMENT OF CL- AND NA+ ACROSS THE TRACHEA OF THE DOG INVITRO

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Abstract
The epithelium of canine trachea maintains a transepithelial potential difference (lumen negative relative to submucosa) by active transport of Cl- toward the lumen and of Na+ away from the lumen. The effects of terbutaline (a .beta.-adrenergic agonist) were examined on short-circuit current, potential difference, electrical conductance and fluxes of 36Cl and 24Na measured across pieces of canine trachea mounted in Ussing type chambers. In short-circuited tissues 10-5 M terbutaline increased unidirectional Cl- flux toward the lumen from 4.78 .+-. 1.12 (mean .+-. SEM) [standard error of the mean] to 6.28 .+-. 1.06 .mu.eq/cm2.cntdot.h. The other measured fluxes did not change significantly. Increases in unidirectional Cl- flux toward the lumen were related to terbutaline concentration (10-7-10-5 M); 10-6 M propranolol prevented or decreased these effects. Apparently terbutaline acts via .beta.-adrenergic receptors to increase active transport of Cl- toward the lumen.

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