Complete nucleotide sequence of sugarcane streak Monogeminivirus

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Abstract
The complete nucleotide sequence of the genome of the Monogeminivirus sugarcane streak virus (SSV) was determined from cloned replicative form DNA. The genome is contained in one DNA circle of 2 758 nucleotides, and has four open reading frames with the potential to encode proteins of MW >10 kDa: two in the viral (+) sense and two in the complementary (−) sense. Each open reading frame has a counterpart among the open reading frames reported for other Monogeminiviruses. A potential binding site for a DNA replication primer and potential transcriptional control sequences were identified on the (+) strand, and a possible intron on the (−) strand. Phylogenetic analysis of coat protein and replication-associated protein sequences of SSV and other grass-infecting geminiviruses indicate that SSV, although distinct from any other virus, is part of an “African streak virus subgroup” of Monogeminiviruses.