A Factor Produced by Cultured Rat Leydig Tumor (Rice-500) Cells Associated with Humoral Hypercalcemia Stimulates Adenosine 3’,5’-Monophosphate Production via the Parathyroid Hormone Receptor in Human Skin Fibroblasts
- 1 June 1985
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 60 (6) , 1144-1147
- https://doi.org/10.1210/jcem-60-6-1144
Abstract
The Rice-500 Leydig cell tumor of Fischer rats is associated with humoral hypercalcemia invivo and produces a factor that stimulates cAMP formation in cultured rat osteosarcoma cells. We found that cultured human skin fibroblasts respond to both human PTH-(1–34) and the factor produced by cultured rat Leydig tumor cells with a dose-dependent rise in cAMP formation. The time courses for stimulation of the two agents were similar, and stimulation by both was blocked by the competitive PTH antagonist [8,18-norleucine,34-tyrosine]bovine PTH-(3–34) amide. These data suggest that PTH-like factors secreted by a murine tumor are capable of interacting with the human PTH receptor.(J Clin Endocrinol Metab60: 1144,1985)Keywords
This publication has 1 reference indexed in Scilit:
- Identification of adenylate cyclase-stimulating activity and cytochemical glucose-6-phosphate dehydrogenase-stimulating activity in extracts of tumors from patients with humoral hypercalcemia of malignancy.Proceedings of the National Academy of Sciences, 1983