Biphasic increase in the in vivo phosphorylation of nuclear 110-kDa protein during early lymphocyte transformation
- 1 May 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 148 (3) , 437-440
- https://doi.org/10.1111/j.1432-1033.1985.tb08858.x
Abstract
A major 110-kDa [kilodalton] phosphoprotein (P 110) in rat liver and hepatoma was identified in nuclear 0.2 M HCl extracts from rat lymph node cells. Stimulation with concanavalin A altered both the amount of P110 and its in vivo phosphorylation in a typical biphasic manner with an initial maximum after 10-14 h. RNA synthesis showed a similar biphasic increase. Inhibition of hn[heterogeneous nuclear]RNA synthesis (5,6-dichlorobenzimidazoleriboside), but not of DNA synthesis (hydroxyurea), markedly depressed both the amount of P110 and its phosphorylation.This publication has 19 references indexed in Scilit:
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