CHARACTERIZATION AND CROSS-REACTIVITY OF HUMAN AND MOUSE ONCOFETAL ANTIGENS USE OF A NEW SOLID PHASE ASSAY FOR DETECTION OF CELL SURFACE ANTIGENS
- 1 February 1982
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 33 (2) , 156-162
- https://doi.org/10.1097/00007890-198202000-00010
Abstract
Oncofetal (OF) antigens were isolated from mouse F9 teratocarcinoma cells, mouse testicular cells, and human molar tissue by detergent extraction followed by dialysis. The soluble antigens were used in solid phase radioimmunoassay (SPRIA) and enzyme-linked immunosorbent (ELISA) assay. Specific antibodies were raised to these antigens in mice. By using these antisera, extensive cross-reactivity was found between mouse and human OF antigens. A human trophoblastic tumor cell line BEWO [choriocarcinoma] absorbed out mouse anti-F9 reactivity. Patients with tumors of germinal origin had antibodies which cross-reacted with mouse and human OF antigens. This new assay is a rapid and sensitive method for the screening of monoclonal antibodies against these antigens and for detecting antibodies to tumors bearing these antigens in patients.This publication has 1 reference indexed in Scilit:
- Carbohydrate structure and cell differentitation: unique properties of fucosyl-glycopeptides isolated from embryonal carcinoma cells.Proceedings of the National Academy of Sciences, 1978