Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis.
- 15 December 1991
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (24) , 11388-11392
- https://doi.org/10.1073/pnas.88.24.11388
Abstract
Ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. Antibodies raised against E1 were affinity purified and used for immunostaining HeLa cells. Condensed chromosomes in mitotic cells were found to be strongly immunoreactive. Chromosomes from metaphase-arrested HeLa cells were isolated and chromosome-associated proteins were analyzed by Western blotting. E1 was detected in fractions containing isolated chromosomes. These results suggest that E1 is associated with condensed chromosomes during mitosis.Keywords
This publication has 30 references indexed in Scilit:
- Immunocytochemical localization of ubiquitin-activating enzyme in the cell nucleusBiochemical and Biophysical Research Communications, 1991
- The Yeast Cell Cycle Gene CDC34 Encodes a Ubiquitin-Conjugating EnzymeScience, 1988
- The gene coding a ubiquitin-activating enzyme may locate on X chromosomeBiochemical and Biophysical Research Communications, 1988
- The yeast DNA repair gene RAD6 encodes a ubiquitin-conjugating enzymeNature, 1987
- Microinjection of ubiquitin: intracellular distribution and metabolism in HeLa cells maintained under normal physiological conditions.The Journal of cell biology, 1987
- Changes in distribution of nuclear matrix antigens during the mitotic cell cycle.The Journal of cell biology, 1984
- A mouse temperature-sensitive mutant defective in H1 histone phosphorylation is defective in deoxyribonucleic acid synthesis and chromosome condensationBiochemistry, 1981
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Evidence for the involvement of H1 histone phosphorylation in chromosome condensationNature, 1980
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970