Folding of the DNA double helix in chromatin-like structures from simian virus 40.
Open Access
- 1 May 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (5) , 1843-1847
- https://doi.org/10.1073/pnas.72.5.1843
Abstract
Relaxed circular, covalently closed simian virus 40 DNA molecules were associated with the four histones that are present in virions. In electron micrographs the resulting complexes appear twisted, with globular structures (nucleosomes) along the DNA. Incubation with an untwisting extract converts the twisted complexes to relaxed structures. Extraction of the DNA from the relaxed complexes yields supercoiled molecules. The number of superhelical turns in these molecules corresponds to the number of nucleosomes per DNA molecule in the complexes.Keywords
This publication has 29 references indexed in Scilit:
- Chromatin Fragments Resembling ν BodiesScience, 1975
- The subunit structure of the eukaryotic chromosomeNature, 1975
- Superhelix density of replicating simian virus 40 DNA moleculesJournal of Molecular Biology, 1974
- Electron microscopy of chromatin subunit particlesBiochemical and Biophysical Research Communications, 1974
- Interphase chromosomal deoxyribonucleoprotein isolated as a discrete structure from cultured cellsJournal of Molecular Biology, 1974
- Chromatin Structure: A Repeating Unit of Histones and DNAScience, 1974
- Dependence of the sedimentation coefficient of denatured closed circular DNA in alkali on the degree of strand interwinding. The absolute sense of supercoilsJournal of Molecular Biology, 1974
- Effect of DNA length on the free energy of binding of an unwinding ligand to a supercoiled DNAJournal of Molecular Biology, 1972
- Interaction between DNA and an Escherichia coli protein ωJournal of Molecular Biology, 1971
- Early and late helix-coil transitions in closed circular DNA the number of superhelical turns in polyoma DNAJournal of Molecular Biology, 1968