Cytochromes P‐450 LM3b and LM4 in Biosynthesis of Bile Acids
- 1 November 1981
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 120 (1) , 29-32
- https://doi.org/10.1111/j.1432-1033.1981.tb05665.x
Abstract
Cytochromes P-450 LM3b and LM4 were prepared from untreated and cholestyramine-treated rabbits. The catalytic properties of these cytochrome P-450 fractions towards substrates in bile acid biosynthesis were studied in reconstituted systems containing NADPH-cytochrome P-450 reductase (EC 1.6.2.4) and phospholipid. Cytochrome P-450 LM3b showed no hydroxylase activity towards cholesterol and only low activity towards some other C27-steroids whereas it catalyzed efficient hydroxylation of testosterone and demethylation of ethylmorphine. Preparations of cytochrome P-450 LM4 catalyzed hydroxylation of cholesterol and other C27-steroids more efficiently than microsomes. Cytochrome b5 had no stimulatory effect on the C27-steroid hydroxylase activities.This publication has 24 references indexed in Scilit:
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