β1,3‐N‐Acetylglucosaminyltransferase‐7 (β3Gn‐T7) acts efficiently on keratan sulfate‐related glycans
- 13 December 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 556 (1-3) , 216-220
- https://doi.org/10.1016/s0014-5793(03)01440-6
Abstract
β1,3-N-Acetylglucosaminyltransferase-7 (β3Gn-T7) has been identified as an anti-migration factor for a lung cancer cell line but its enzymatic activity has not yet been characterized. Here we show that β3Gn-T7 efficiently acts on keratan sulfate-related glycans including Galβ1→4(SO3−→6)GlcNAcβ1→3Galβ1→4(SO3−→6)GlcNAc (L2L2), while lacto-N-tetraose and lacto-N-neo-tetraose were poor substrates. Moreover, we found that among the other five β3Gn-Ts and i antigen-producing β3Gn-T (iGn-T), β3Gn-T2 and iGn-T act well on L2L2, although these specific activities were lower than those for a tetraantennary N-glycan. These results indicate that β3Gn-T7 is the one that most efficiently elongates L2L2 and may be involved in the biosynthesis of keratan sulfateKeywords
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