Conversion of (—)-Menthone byPseudomonas fluorescensM-2

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Abstract
A microorganism M–2 was isolated as a strain capable of converting (—)-menthone to other compounds. The strain was identified as Pseudomonas fluorescens by taxonomical investigation. The conversion products of (—)-menthone were determined to be (—)-t-4-isopropyl-3-oxo-r-l-cyclohexanecarboxylic acid,* (+)-c-4-isopropyl-3-oxo-r-1-cyclohexane-carboxylic acid* and (+)-t-3-hydroxy-t-4-isopropyI-r-l-cyclohexanecarboxylic acid.* As the main pathway, it was proposed that (—)-menthone was oxidized to a keto acid which was successively reduced to a hydroxy acid.

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