Long-term storage of eggs of Simulium ornatum

Abstract
A method for the storage of non‐diapausing eggs of Simulium ornatum Meigen has been developed. High survival rates for 5–6 months depend on the age of eggs when placed at reduced temperature. A period of precooling or conditioning is required for 28 days before storage of the eggs at 1oC with lowering of oxygen partial pressure. Induced quiescence does not appear to affect development from egg to adult.