Use of whole-cell fixation to visualize replicating and maturing simian virus 40: identification of new viral gene product.

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Abstract
Formaldehyde fixation of SV40-infected CV-1 [African green monkey kidney] cells at appropriate times after infection permits isolation of crosslinked complexes of SV40 minichromosomes during the time of DNA replication and during packaging with viral proteins. Such crosslinked complexes can be separated on the basis of density on CsCl/guanidine.cntdot.HCl density gradients. During the course of these studies the presence of a low MW protein in a region of the gradient much enriched with viral nucleoproteins was observed. This protein is present only in infected cells and has a MW and amino acid composition consistent with it being the product of the so-called SV40 agnogene.